Download PDF Amoebic meningo-encephalitis

By Alina Ellerington, Encephalitis Society

What are amoebas?

Amoebas are single-celled organisms usually found in water, wet soil, rotting vegetation, animals and humans. Although most types are harmless, a few of them such as Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri and Sappinia pedata can produce life-threatening diseases in humans such as GAE and PAM. These diseases are difficult to diagnose and probably under reported. Most of the time, the diagnosis is made post-mortem (after death).

Amoebas enter into the human body through nasal or cutaneous (skin) route. Recently, organ transplantation become a new mode of entry. A recent study claims that the brain damaged in these types of encephalitis is caused by the immune system, which overreacts rather than the amoeba itself.

Granulomatous amoebic encephalitis (GAE)

Acanthamoeba spp, Balamuthia mandrillaris and Sappinia pedata cause encephalitis of a granulomatous type that develop gradually over an extended period of time.

Acanthamoeba spp is the most common amoeba found in a wide range of locations: soil, water, air, dust, ventilation and air conditioning systems. In addition, it has been found in hospitals, medicinal pools, dental treatment units, dialysis machines and contact lenses.

Despite the many opportunities for contact with amoeba, the Acanthamoeba infections are rare in humans and, except for the Acanthamoeba keratitis (AK), it mostly occurs in immunocompromised individuals (those having an immune system weakened by the illness or treatment). Acanthamoeba enters the body through lesions in the skin contaminated by the soil or through upper respiratory tract (mouth or nose). Most people have a history of skin lesion or lung infection occurring a few months earlier.

Once in the body, amoeba can spread to the central nervous system (CNS) and various organs. The types of infections produced include GAE, nasopharyngeal, cutaneous and disseminated infections and AK when Acanthamoeba directly attacks the corneal surface (eyes). Symptoms can include fever, headache, behavioural manifestations and hemiparesis. Acanthamoeba is not usually found in cerebrospinal fluid (CSF) but it can be isolated through brain biopsy. It is usually misdiagnosed hence the high mortality rate.

The treatment consists in various antimicrobial drugs (drugs to treat the infection) sometimes used in the same time. Drugs that result in a cure in one patient may have no success in other patients. The outcome is influenced by the following factors: early treatment, the infective dose of amoeba, the type and characteristics of amoeba, and the immune system of the individual. Most survivors had skin lesions that helped in the early detection of GAE. Their treatment consisted in both surgical resection of the affected lesion and multiple antibiotics.

Balamuthia amoebic encephalitis (BAE)

Balamuthia mandrillaris, a newest discovered type of amoebae, can cause Balamuthia Amoebic Encephalitis (BAE), which leads to death in most of the cases. Balamuthia is present in soil and possibly water although there was no obvious history of swimming or other water activities for individuals who have developed BAE. The contamination happens through respiratory route or skin lesions (breaks in the skin).

There are approximately 100 cases of BAE published globally with half of them being in the USA. The earliest cases presented involved immunocompromised individuals, but more recently, cases involving children are published.

Patients present with headache, fever, characteristics skin lesions, stiff neck, nausea, vomiting, acute confused state, aphasia (impairment of language) and seizures. Though the course of the illness is gradual, the disease progresses within weeks to months. The diagnosis is made most of the time after death through autopsy. The treatment is problematic, as there is no antimicrobial treatment protocol determined yet.

Only three survivors of BAE caused by Balamuthia have been reported. Two of them (a five year old girl and 64 year old man) survived due to an early diagnosis and rapid initiation of antimicrobial treatment. The diagnosis in both cases was confirmed through the brain biopsy.  Both patients were doing well at the follow-up, two and six years after the onset of the symptoms.

Primary amoebic meningo-encephalitis (PAM)

Naegleria fowleri (‘brain eating amoeba’) produces primary amoebic meningoencephalitis (PAM). This is a very rare, but serious disease with a mortality rate of more than 95%. From 1962 up to present, there were 133 cases reported in the USA with only 3 survivors and overall 250 cases worldwide.

Naegleria fowleri is more sensitive to the environment than acanthamoeba and it cannot survive in seawater. There are no reports of Naegleria fowleri being present in Antarctica. Contamination can happen through swimming in contaminated fresh water or through nasal irrigation for cleansing (religious practice, medical indication or recreational play). Warm fresh waters (lakes, rivers and hot springs) together with a bacterial food supply are ideal habitats for Naegleria fowleri. Usually, it affects healthy children and young people, more males than females, who have a history of swimming or bathing in fresh water. Most people have become infected during periods of very warm temperatures.

After entering the nostrils, Naegleria fowleri circulates towards the brain along the olfactory nerves tract. It develops within a few days of contamination, with fever, headache, behavioural disturbances, nausea and vomiting. Death occurs 1-2 weeks after hospitalisation. The dramatic outcome could be a result of the rapid onset and delay in diagnosis.

Treatment consists of a mixture of drugs ranging from antimicrobial drugs to experimental anti-cancer drugs. Naegleria spp. are highly sensitive to the antifungal drug amphotericin B (AmB), and it has been used in all cases as the core antimicrobial where recovery occurred.

The level of Naegleria fowleri in swimming water should be controlled. In 2000, Australia adopted a legal standard for Naegleria fowleri in surface water: a guideline level of five thermophilic Naegleria fowleri per litre, and an action level of two thermophilic Naegleria fowleri per litre.

Greater awareness of these amoebas and the diseases they cause could improve the timescale to reach a diagnosis before death occurs. This together with an effective antimicrobial treatment may improve the chances of survival for people with GAE or PAM.

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FS045V1 Amoebic meningo-encephalitis

Date created: December 2015; Review date: December 2018

Disclaimer: We try to ensure that the information is easy to understand, accurate and up-to-date as possible. If you would like more information on the source material the author used to write this document please contact the Encephalitis Society. None of the authors of the above document has declared any conflict of interest which may arise from being named as an author of this document.